Ultrastructure of Chloroplasts Isolated by Nonaqueous Extraction

INTRODUCTION been carried out with anthocyanin as a marker of vacuolar material and with various enzymes as markers of mitochondrial and hyaloplasmic commethod of isolating cell organelles (2) and its markers of mitochondrial and hyaloplasmic commodification for use in isolating chloroplasts (3, ponents 5, 13), it has been used extensively in the study visual observations of the purity of these preparaof the intracellular distribution of water-soluble tions and the structure of these plastids have enzymes, minerals, and photosynthetic reaction been confined to light microscopy (16). Where products (4, 6-16). electron microscopy has been used, the isolated Tests of the purity of isolated chloroplasts have plastids generally have been prepared for study